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. 2015 Oct 16;19(12):2751–2762. doi: 10.1111/jcmm.12703

Figure 1.

Figure 1

Impaired mast cells (MCs) are associated with deterioration of cardiac function in Kit W/W‐v mice after ischaemic injury. (A) Wild‐type (WT) and Kit W/W‐v mice underwent myocardial infarction and received isogenic (WT and Kit W/W‐v) MCs or medium alone. Per cent fractional shortening was evaluated by echocardiography before (day −3) and after (days 7 and 14) infarction (n = 5, *P < 0.05 for WT MCs versus WT medium‐injected; ##P < 0.01 for Kit W/W‐v MCs versus WT MCs). (B) Whole heart serial sections demonstrated increased scar size 14 days after implantation with isogenic MCs in Kit W/W‐v mice compared to WT recipients of WT MCs (n = 5, **P < 0.01 versus WT). (C) An MTT assay demonstrated that co‐culture with WT MCs increased WT fibroblast (FB) proliferation compared to co‐culture with Kit W/W‐v MCs (n = 3, ***P < 0.001 versus WT). (D) A gel contraction assay demonstrated co‐culture with WT MCs increased the contractile function of WT FBs, while co‐culture with Kit W/W‐v MCs did not (n = 3, ***P < 0.001 versus FBs). Gel contraction is expressed as the per cent shrinkage compared with a control gel without cells.