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. Author manuscript; available in PMC: 2015 Dec 22.
Published in final edited form as: Science. 2015 Mar 19;348(6233):442–444. doi: 10.1126/science.aaa5945

Figure 2.

Figure 2

Experimental demonstration of MCR in Drosophila. A) Mendelian male inheritance of an X-linked trait. B) Theoretical MCR-based inheritance results in the initially heterozygous allele converting the second allele generating homozygous female progeny. C) Diagram of y-MCR construct. Two y locus homology arms flanking the vasa-Cas9 and y-gRNA transgenes are indicated and the locations of the PCR primers used for analysis of the genomic insertion site (listed in the Methods section). D) PCR analysis of a y+ MCR-derived F2♂ (lanes 1–3, see Fig. S1 for sequence), yMCR F1♀ (lanes 4–6) and ♂ (lanes 7–9) showing junctional bands corresponding to y-MCR insertion into the chromosomal y locus (lanes 2–3,5–6,8–9) and an approximately wild-type size band from the y locus (lanes 1,4,7). Although the yMCR F1♂ (carrying a single X-chromosome) displays only MCR derived PCR products (lanes 8–9), yMCR F1♀s generate both MCR and non-insertional amplification products. E) Summary of F2 progeny obtained from crosses described in detail in Table S1. F) Low magnification view of F2 progeny flies from an yMCR X y+. Nearly all female progeny display a y- phenotype. G) High magnification view of a full body yMCR F1♀. H) A rare 50% left-right mosaic female. I) A y+ control fly.