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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Technology (Singap World Sci). 2015 Jun 23;3(4):179–188. doi: 10.1142/S2339547815500065

Figure 4.

Figure 4

Microfluidic 3D co-culture of human BMSCs and PC3 prostate cancer cells. (a) Schematic of PC3 human prostate cancer cell seeding into a scaffold-chip and subsequent perfusion culture. (b) Fluorescent image of Luc-GFP PC3 prostate cancer cells captured in a microfluidic scaffold after 3 days perfusion culture. PC3 cells are red colored to distinguish from human BMSCs. (c) Schematic of human BMSCs seeding into a scaffold-chip by single rapid injection and subsequent perfusion culture. (d) 3D confocal images of post 3 days perfusion culture show that BMSCs stably adhered on pore surface and well distributed both axial and lateral directions of an integrated hydrogel scaffold. (e,f) Fluorescent images of co-culture microfluidic chip show engrafted human prostate tumor cells in 3D microenvironment.