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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Technology (Singap World Sci). 2015 Jun 23;3(4):179–188. doi: 10.1142/S2339547815500065

Figure 5.

Figure 5

Retrieval of hydrogel scaffold from microfluidic devices with retained xenograft capacity. (a) Schematic of retrieval of an integrated hydrogel scaffold from a microfluidic device for continues in vitro and in vivo analysis. (b) In vitro growth analysis of microfluidically captured Luc-GFP PC3 prostate tumor cells after transferring to a well plate using bioluminescent imaging (n = 6, *p < 0.05). Fluorescent image of tumor cells in a retrieved scaffold (Inner panel). (c) Morphological analysis of tumor cell growth with and without human BMSCs after 3 weeks in vitro culture. (d) In vivo growth analysis after subdermal implantation into immunodeficient mice using bioluminescent imaging. (e) Quantification of bioluminescent monitoring of tumor cell growth over 5 weeks in vivo (n = 6). (f) Gross image of explanted scaffolds 7 weeks after implantation.