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. 2015 Oct 16;34(24):3009–3027. doi: 10.15252/embj.201591006

Figure EV1. Rpp30 protein expression is affected in Rpp30 18.2 mutants and PCNA loss in Rpp30 18.2 ovaries is restored when overexpressing ubiRpp30GFP (related to Figs 1 and 3).

Figure EV1

  • A
    Normalized fold change and P‐value between Rpp30 18.2 mutant ovaries and white virgins issue from RNA‐seq transcriptome analysis showing a downregulation of Rpp30 transcripts in Rpp30 18.2 mutant ovaries.
  • B, C
    flp/FRT clones mutant for Rpp30 18.2 specifically in the germ line (B) or follicular cells (C) are detected by the absence of RFP or GFP, respectively. Magnifications show Rpp30 absence in the nucleus of mutant cells. Perinuclear Rpp30 foci are indicated by white arrows in a wild‐type nurse cell (B). Scale bar, 10 μm.
  • D
    Rpp30 18.2 homozygous ovaries overexpressing ubiRpp30GFP were dissected, fixed, and stained for PCNA (red). Rpp30GFP is in green and DAPI is in blue. Scale bar, 10 μm.