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. 2015 Oct 16;34(24):3009–3027. doi: 10.15252/embj.201591006

Figure 3. Rpp30 mutation leads to the activation of several checkpoint proteins and to replication stress.

Figure 3

  1. Rpp30 18.2 homozygous early oogenesis arrest is partially rescued by p53 mutation. Scale bar, 100 μm.
  2. Early oogenesis arrest found in Rpp30 18.2 homozygous ovaries is rescued by chk2 mutation (Rpp30 18.2, mnk p6 and Rpp30 18.2 /Rpp30 PE , mnk p6). Scale bar, 100 μm. Asterisk: example of one rescued stage 9 egg chamber. Arrow: example of non‐rescued egg chambers.
  3. Germ line clones mutant for Rpp30 18.2 were immunostained for PCNA (red). DAPI is in blue. Magnifications: PCNA signal in a control or mutant nurse cell. Scale bar, 10 μm.
  4. Germ line clones mutant for Rpp30 18.2 were immunostained for Brf (pol III) (red). DAPI is in blue. A Z‐projection of Brf staining is shown. The arrow points to Brf aggregates in a mutant chamber. Scale bar, 10 μm.
  5. Early oogenesis arrest found in Rpp30 18.2 homozygous ovaries is partially rescued by claspin mutation (Rpp30 18.2 ; claspin EP /TM3). Scale bar, 100 μm.
  6. Quantification of rescued ovaries harboring at least one stage 9 egg chamber in genotypes numbered from 1 to 11 after 1 day or several days on yeast. Gray bars (1–4) are control flies. n = number of ovaries.
  7. Upper panel: Early oogenesis arrest found in Rpp30 182./PE transheterozygous ovaries is rescued by sqd‐S‐HA overexpression. Scale bars: 100 μm. Lower panel: Rpp30 18.2/PE; sqd‐S‐HA/sqd‐S‐HA ovaries were dissected and stained for Orb (green), HA (squid, red), and DAPI (blue). Scale bar, 10 μm.

Source data are available online for this figure.