Figure EV5. Chk2 activation by spnA mutation does not recapitulate Rpp30 phenotype (related to Figs 3, 5, and 6).

1. RNA extracted from heterozygous or homozygous spnA ¹ and spnE ¹ flies was used to study transposable element expression by RT–qPCR. The fold change of TEs expression comparing homozygous versus respective heterozygous ovaries is shown.
2. Ovaries from homozygous spnA ¹ and spnE ¹ flies were fixed and stained for Aub (left) or PCNA (right). Scale bar, 10 μm.