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. 2015 Dec 22;10(12):e0145765. doi: 10.1371/journal.pone.0145765

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© 2015 Mendes et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) The lanes contain one positive control, two negative controls, and the Xf-VapD samples that were treated at 37°C and 90°C. The values are shown in RFU/time units. The curves show that Xf-VapD demonstrated ribonuclease activity at both 37°C, and after heating to 90°C. (B) Lane 1 contains RNA alone, and lanes 2 to 5 contain RNA with Xf-VapD (1 μM, 5 μM, 10 μM and 20 μM, respectively). (C) Lane 1 contains dsDNA (2 kb) alone, and lanes 2 to 3 contain dsDNA (2 kb) with Xf-VapD (20 μM and 50 μM, respectively). (D) Lane 1 contains dsDNA (20 bp) alone, and lanes 2 to 3 contain dsDNA (20 bp) with Xf-VapD (20 μM and 50 μM, respectively). (E) Lane 1 contains ssDNA (26 nt) alone, and lane 2 contains ssDNA (26 nt) with Xf-VapD (50 μM).