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. 2015 Dec 22;10(12):e0145762. doi: 10.1371/journal.pone.0145762

Fig 1. Characterization of single TCR T cell mice.

Fig 1

A) Splenocytes were collected from WT and single TCR T cell C57BL/6 mice and analyzed by flow cytometry for co-expression of Vα2 with Vα3.2 or Vα8.3 (top panels) or co-expression of Vβ6 with any of a panel of fourteen other Vβ proteins (bottom panels) in CD3+ CD4+ T cells. Representative flow plots from three independent experiments show the presence of dual TCRα and β populations in WT (boxed populations in left panels) that are absent in single TCR T cell mice (right panels). B) Flow cytometric analysis of splenocytes from WT and single TCR T cell mice reveals equivalent CD3 expression among CD4+ and CD8+ T cells. C) Developmental T cell stages (left) and peripheral T cell subsets (right) from WT and single TCR T cell mice were analyzed and enumerated by flow cytometry (n = 6). D) The number of γδ T cells in the lymph nodes and spleen of adult WT or single TCR T cell mice as determined by flow cytometry (B220-, CD11b-, CD11c-, CD3+ and TCRγδ+). Results shown are mean +SEM (n = 3 for TCRα+/- and TCRα+/- TCRβ+/-, n = 2 for TCRβ+/-, and n = 6 for WT). Student’s t-test was used to determine p values; ***p<0.001. Flow cytometry plots are in Log10 fluorescence scale.