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. 2015 Dec 22;10(12):e0145762. doi: 10.1371/journal.pone.0145762

Fig 3. Bi-allelic TCRα and β chain recombination affect thymic selection efficiency.

Fig 3

T cell-depleted bone marrow was collected from WT C57BL/6 (CD45.1 and CD90.2) mice and combined with that of either TCRα+/-, TCRβ+/-, or TCRα+/- TCRβ+/- (all CD45.2 and CD90.2) mice in equal proportions and injected into lethally-irradiated C57BL/6 CD90.1 mice. Thymus, spleen, and lymph nodes were collected 10 weeks following transplantation. The ratios of WT:TCRα+/-, WT:TCRβ+/-, and WT:TCRα+/- TCRβ+/- (CD45.1:CD45.2) cells were determined by flow cytometric analysis of A) thymocytes at the indicated developmental stages and B) peripheral lymphocyte subsets. All ratios are adjusted to the peripheral B cell CD45.1:CD45.2 ratio in each mouse, as an internal control. The data plotted include the mean and SEM (n = 7–8 mice/group). Student’s t-test was used to determine p value, relative to the WT:WT control. *, **, and *** indicate p<0.05, p<0.01, and p<0.001 respectively. Student’s t-test was used to determine p values of CD45.1:CD45.2 ratios pre/post α and β (DPpre/DPpost and DN1/DPpre respectively). ††† indicates p<0.001 for pre/post selection comparisons.