Skip to main content
. 2015 Dec 22;10(12):e0145102. doi: 10.1371/journal.pone.0145102

Fig 1. Conditional deletion of apoE in mice using Cre/loxP system.

Fig 1

(A) Scheme of the Apoe allelels flanked by loxP sites. (B,C) Apoe gene expression level of generated knockout models of either (B) liver of Apoe ΔHep mice (n≥8) and (C) adipose tissue of Apoe ΔAT mice (n≥6). (D) Total apoE protein amount of liver lysates of Cre- and Cre+ mice crossed with either wt/Apoe flox or Apoe flox/flox mice as indicated. ApoE protein was detected at 34kDa by Western blot (n≥3). (E) ApoE plasma levels of Apoe ΔHep and Apoe ΔAT mice after feeding chow, a diabetogenic high fat diet (HFD or a cholesterol-containing Western type diet (WTD). ApoeΔHep and respective controls on chow (n≥5), HFD (n≥8) and WTD (n≥6); ApoeΔAT and respective controls on chow (n≥7), HFD (n≥8) and WTD (n≥3). WT: Wild-type. WAT: white adipose tissue. Mean values ± s.e.m. Statistical analysis with Students T-Test. *: p<0.05.