Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 21;211(6):1131–1140. doi: 10.1083/jcb.201503070

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Saroufim et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — mRNAs scan the nuclear periphery before export to the cytoplasm. (A) Localization of CLB2, MDN1, and polyA mRNA by FISH suggests a rate-limiting step at the nuclear periphery. Blue arrows show sites of transcription. Yellow arrows show single mRNAs. DNA is stained with DAPI (blue). (B) Cartoon illustrating the mRNA labeling strategy using PP7 stem loops. (C) Live cell imaging of CLB2 mRNA. Individual frames from video acquired in 37-ms intervals. MAX shows the maximum intensity projection of all frames. mRNA is shown in green and NPC in red. The yellow arrows show single tracked mRNA in each frame. The purple arrow outlines the same RNA path in the MAX projected image. (D) Track of nuclear CLB2 mRNA from C and Video 1. The RNA position in each frame was determined using 2D Gaussian fitting and was superimposed onto a single frame of Video 1 (middle). Left panel shows connected positions to visualize the path from the nucleus to the cytoplasm.