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. 2015 Dec 21;211(6):1131–1140. doi: 10.1083/jcb.201503070

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© 2015 Saroufim et al.

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Figure 4. — mRNP–NPC interactions mediate perinuclear scanning. (A) Cartoon describing the relationship between the nuclear polyA RNA binding protein Nab2 and the C-terminal domain of Mlp1p. (B) Localization of the Mlp1ΔC-2xmCherry fusion protein to the nuclear periphery. See text for details. (C) Deletion of the C terminus of Mlp1 does not affect basket integrity. Coomassie-stained gel separating protein complexes isolated by single-step affinity purification using Mlp1-ProtA, Mlp1ΔC-ProtA, or ProtA as baits. White line indicates that intervening lanes have been spliced out. Table with normalized peptide counts of copurified proteins as determined by mass spectrometry. Only selected NPC components are shown; for full list, see Table S2. (D) Quantification of GAL1pro-24PP7-GLT1 mRNP scanning behavior in mlp1ΔC and nab2F73D. 156 (WT), 75 (Mlp1-ΔC), and 85 (Nab2 F73D) tracks were analyzed. (E) Frequency of static frames at the periphery for GAL1pro-24PP7-GLT1 mRNPs in Δtom1 strain. P < 0.05, comparing WT versus mutants using a randomized ANOVA followed by posthoc tests, except WT versus Mlp1ΔC for scanning.