Figure 1.

Mouse primary B cells repair SLO-induced plasma membrane wounds in a Ca²⁺-dependent manner. Primary mouse B cells were incubated with SLO (0–400 ng/ml) at 4°C for 5 min and warmed up to 37°C for 5 min in medium with (+Ca²⁺) or without Ca²⁺ (−Ca²⁺), followed by PI staining at 4°C. The percentage of PI positive (PI⁺) cells was quantified as nonrepaired cells using flow cytometry (A) and normalized to untreated cells (B). The percentage of cells repaired was calculated as: [%PI⁺ (−Ca²⁺) − %PI⁺ (+Ca²⁺)] × 100/%PI⁺ (−Ca²⁺; C). Shown are representative histograms (A) and the mean (± SD; B and C) of three independent experiments. *, P < 0.05; **, P ≤ 0.0005.