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. 2015 Dec 21;211(6):1113–1120. doi: 10.1083/jcb.201507006

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© 2015 Gluszek et al.

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Figure 1. — Sentin is required to separate homologous centromeres in oocytes. (A) Meiotic progression in Drosophila oocytes. The arrowhead indicates a chromosome protrusion. (B) Immunostaining of mature oocytes. (C) Frequency of Msps and D-TACC concentrated at the spindle poles, and bipolar spindles in wild-type (control) and sentin mature oocytes (n = 13, 36, 24, 58, 95, and 66). The error bars of the spindle length indicate the SEM (n = 94 and 120). Other error bars indicate the 95% confidence intervals. **, P < 0.01; ***, P < 0.001, significant differences between wild type and sentin. (D) FISH in oocytes using a dodeca-satellite probe specific to centromere 3. The arrowheads indicate centromere 3 signals. (E) The distances between homologous centromeres from FISH probed by dodeca satellite (cen 3) and AACAC satellite repeat (cen 2). **, P < 0.01; ***, P < 0.001, significant differences from the medians of wild-type control. Bars, 10 µm.

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