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. 2015 Dec 21;211(6):1113–1120. doi: 10.1083/jcb.201507006

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© 2015 Gluszek et al.

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Figure 3. — Sentin suppresses precocious stable kinetochore–microtubule attachments. (A) Time-lapse images of oocytes expressing GFP-Mis12 and Rcc1-mCherry. The numbers are in minutes from full recruitment of Mis12. (B) Kymographs of the same oocytes with the long axis of the spindle against time (Y-axis). (C) Time-lapse images of oocytes expressing Rod-GFP and Rcc1-mCherry. The numbers are in minutes from full recruitment of Rod. Arrowheads indicate Rod-GFP streams. (D) Kymographs of the same oocytes. (E) The frequency of weakly (open bars), moderately (half-filled), and strongly (filled) attached kinetochores judged by Rod-GFP (Materials and methods). For each genotype, a total of 20–32 kinetochores in five oocytes were followed. *, P < 0.05; ***, P < 0.001, significant differences from the control. Error bars indicate the 95% confidence intervals of the proportion of attached kinetochores. (F) The approximate timing of cytological events in oocytes. KT, kinetochore; MT, microtubule; NEB, nuclear envelope breakdown; Time 0, time when full recruitment of kinetochore proteins, Mis12 and Rod, has occurred. Bars, 10 µm.

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