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. 2015 Dec 21;211(6):1141–1156. doi: 10.1083/jcb.201508072

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© 2015 Samejima et al.

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Figure 8. — Effects of depleting CENP-O,-P,-Q,-R,-U from chromosomes. (A) Scatterplot of H/L ratios from two replicate experiments with the CENP-O^KO. (B) Enlargement of the blue box in A. Levels of most CCAN proteins (red) are increased in CENP-O^KO chromosomes. (C) Profile plot shows the behavior of CENP-O group proteins in all experiments. (D) Scatterplot of H/L ratios from two replicate experiments with the CENP-R^KO. Enlargement of the green box confirms the increase in levels of most CCAN proteins, whereas the red box reveals a decrease in RZZ-associated proteins. wt, wild type.