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. 2015 Dec 22;10(12):e0145630. doi: 10.1371/journal.pone.0145630

Fig 5. Expression of histone methyltransferase Suv39h1 and Suv39h2 in MDGC cells.

Fig 5

(A) Expression of histone methyltransferases for H3K4 (Mll1, Mll2, Mll3, Mll4, Setd1a and Ash1l), H3K9 (Suv39h1, Suv39h2, G9a and Setdb1), and H3K27 (Ezh2) was determined by RT-PCR. The expression levels of Suv39H1 and Suv39H2 were inversely correlated with Twist1 expression. Gapdh was used as an internal control. (B and C) Effect of Suv39h1 or Suv39h2 knockdown on Twist1 expression in MDGC cells. RT-PCR was performed on MDGC-1 and MDGC-3 cells with Twist1 expression after transfection of siSuv39h1 (sih1), siSuv39h2 (sih2) or negative control (siNC) siRNA. (C) The Twist1, Suv39h1 and Suv39h2 mRNA levels in MDGC-1 cells were further confirmed by qRT-PCR. The columns and bars indicate averages and S.D., respectively. **P<0.01.