Table 1. Frequency of Twist1 methylation in DGC from DCKO mice.
| Sample type | Total | Methylation frequency(%) | IHC expression 5) | |||||
|---|---|---|---|---|---|---|---|---|
| ++ | + | — | frequency(%) | |||||
| Normal gastric mucosa 1) | 3 | 0 | (0) | ND | ND | ND | ND | |
| Non-cancerous gastric mucosa bearing GCs 2) | 10 | 1 | (10) 6) | 0 | 0 | 10 | (0) 7) | |
| GCs | 18 | 9 | (50) 6) | 3 | 4 | 11 | (38.9) 7) | |
| Intramucosal GCs 3) | 10 | 4 | (40) | 1 | 2 | 7 | (30) | |
| Invasive GCs 4) | 8 | 5 | (62.5) | 2 | 2 | 4 | (50) | |
1) Gastric mucosa from control mice (Atp4b-Cre −; Cdh1 loxP/loxP; Trp53 loxP/loxP mice)
2) Gastric mucosa from DCKO mice bearing GCs
3) GC confined to the mucosa or submucosa
4) GC invades the muscularis propria, subserosa or serosa
5) The intensity of nuclear staining was scored as + (weak), and ++ (moderate to strong) in more than 10% of the tumor cells. The frequencies of Twist1 positive (++, +) are calculated.
6) p < 0.05 Fisher's exact test
7) p < 0.05 Fisher's exact test