Table 3.
Validation parameters of the developed method for the determination of melamine and cyromazine in egg yolk.
| VALIDATION PARAMETERS | MELAMINE | CYROMAZINE |
|---|---|---|
| Linear range mg/kg | 2.5–30 | 2–30 |
| Linearity R2 | 0.9998 | 0.9931 |
| Linear equation (y = peak area, x = mg/kg) |
y = 37.0x + 771312 | y = 28.1x − 101706 |
| LOQ S/N = 10 (mg/kg) | 2.5 | 2 |
| Intra-day precision and accuracy% | 75–110% | 75–79% |
| RSD% n = 3 levels × 3 samples | RSD < 4% | RSD < 7% |
| Inter-day precision and accuracy% | 82–108% | 76–116% |
| RSD n = 3 levels × 3 samples × 5 days | RSD < 3.5% | RSD < 10% |
| Recovery% | 74% | 75% |
| CCalfa (mg/kg) | 2.6 | 2.0 |
| CCbeta (mg/kg) | 2.8 | 2.2 |