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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Neurogastroenterol Motil. 2015 Oct 13;28(1):64–73. doi: 10.1111/nmo.12697

Figure 1.

Figure 1

NaV1.5 is present in rat GI tract. A) Protein immunoblots from rat heart, jejunum and colon tunica muscularis demonstrate expression of Nav1.5. B) and C) Immunoreactivity for Nav1.5 in the muscularis propria of rat jejunum. Immunolabeling was detected in the longitudinal (LM) and circular (CM) muscle layers using both goat (Gt) α Nav1.5 antibody (B) and rabbit (Rb) α Nav1.5 (C) antibodies directed against different epitopes on the Scn5a protein (upper panels). No signal is detected when the tissues were incubated with only the secondary antibodies (lower panels). Tissues were labeled at the same time and images were collected with the same exposure times.