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. 2015 Dec 6;13(10):2118–2125. doi: 10.1016/j.celrep.2015.11.012

Figure 4.

Figure 4

Knockdown of Myosin 1b, 9b, 10, and 18a Affects Focal Adhesion and Actin and NM2A Organization in PC-3 Cells

(A and B) Control cells treated with non-targeting siRNA and myosin-depleted cells co-stained for F-actin (red) and NM2A (green) (A). An enlarged view of the region in the boxed area in (A) is shown in (B). Arrowheads indicate F-actin structures in KD cells. Scale bars, 5 μm (A) and 2 μm (B).

(C) Control NT cells and myosin-depleted cells co-stained for F-actin (red) and focal adhesions using paxillin (green). Arrowheads indicate focal adhesions at the ends of F-actin bundles in KD cells Scale bar, 30 μm.

(D) Immunoblotting for phosphorylated myosin light chain (pMLC) and ERK or GAPDH (loading control) in control and KD cells. Quantification of the control and Myo9b KD blot shown here showed a 1.8-fold increase in pMLC in KD compared with NT cells. UT, untreated.