Skip to main content
. Author manuscript; available in PMC: 2016 Dec 17.
Published in final edited form as: Mol Cell. 2015 Nov 25;60(6):953–965. doi: 10.1016/j.molcel.2015.10.029

Figure 5. Comprehensive analysis of productive slippage synthesis.

Figure 5

A. Nucleotide addition cycle for the standard pathway of transcription initiation. Left: initial transcribing complex with a 2-nt RNA in a pre-translocated state. Middle: initial transcribing complex with a 2-nt RNA in a post-translocated state. Right: 3-nt product complex in a pre-translocated state. The RNA and DNA template strand remain in lock-step register and the sequence of the RNA is fully complementary to the template strand. White boxes, DNA; blue boxes, RNA; gray shading, RNAP; red, TSS bases; i and i+1, RNAP active-center i and i+1 sites.

B. Nucleotide addition cycle for the slippage pathway. Left: initial transcribing complex with a 2-nt RNA in a pre-translocated state. Middle: RNA has moved backward relative to the DNA template by one base. Right: 3-nt product complex in a pre-translocated state. The 5′ end of the RNA carries an RNA/DNA difference and is not complementary to the template strand.

C. Analysis of productive slippage synthesis. Graphs show % slippage (mean + SEM) for TSS-region sequences containing 5′ end homopolymeric repeat sequences of the indicated length that begin at the indicated position (TSS).

(See Figure S5)