Figure 6. Effects of NTP concentrations on transcript yields in vitro.
A. and B. Relative expression histograms for experiments performed at saturating NTP (A) and non-saturating (B) NTP concentrations using a non-supercoiled linear DNA template in vitro. Relative expression for TSS-region sequences with ≥ 25 total RNA reads for which the number of DNA templates was not in the top or bottom 10% (Tables S4 and S5). For each experimental condition the lowest value of relative expression was normalized to 1.
C. Normalized relative expression for the indicated TSS-region sequences. Values were calculated by dividing the average relative expression for the indicated TSS-region sequence by the relative expression observed for all TSS-region sequences.
(See Figure S6)