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. Author manuscript; available in PMC: 2016 Dec 28.
Published in final edited form as: J Control Release. 2015 Jul 23;220(0 0):600–607. doi: 10.1016/j.jconrel.2015.07.019

Fig. 2.

Fig. 2

(A) Schematic of the preparation of RBC-NPs with targeting ability. Lipid-tethered ligands are synthesized and then inserted into RBC membrane ghosts, resulting in ligand-functionalized RBC membranes which are ultimately used to coat polymeric cores to form targeted RBC-NPs. (B) Fluorescence microscopy imaging of KB cells incubated with folate-functionalized RBC-NPs, non-targeted RBC-NPs, and folate-functionalized RBC-NPs together with free folate. The targeted RBC-NPs demonstrated significantly higher uptake by cells overexpressing the folate receptor. Scale bars = 25 µm. (C) Fluorescence microscopy imaging of MCF-7 cells incubated with AS1411-functionalized RBC-NPs, non-targeted RBC-NPs, and AS1411-functionalized RBC-NPs together with free AS1411 aptamer. The AS1411- functionalized RBC-NPs demonstrated significantly higher uptake in cells overexpressing nucleolin. Scale bars = 25 µm. Reproduced with permission from Ref. [53].