Skip to main content
. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Neurobiol Dis. 2015 Oct 13;85:130–143. doi: 10.1016/j.nbd.2015.10.003

Figure 3. Aggregation and hydrophobicity of Bri1-23 pE/E, ADan pE/E, and ADan1-28 pE.

Figure 3

(A) Oligomerization/fibrillization was assessed by fluorescence evaluation of ThT binding to the respective synthetic homologues (50 μM) over 24 hours. (B) SDS-PAGE. Peptides (20 μM) were incubated at 37°C for up to 24 hours and samples at different time-points were separated in a 16.5% Tris-tricine SDS gel followed by WB analysis. All blots were probed with the anti-ADan 5282 antibody (1:5,000). (C) Exposure of hydrophobic regions of the molecules was evaluated by assessing the binding of the peptides to ANS. In A and C, results are expressed in arbitrary units (A.U.); in both cases, data represent the mean ± SEM of three independent experiments after subtraction of blank levels.