Skip to main content
. 2015 Dec 22;5:81. doi: 10.1186/s13568-015-0173-9

Fig. 2.

Fig. 2

Direct PCR analysis of genomic DNA recovered from individual transformants. Supernatants of homogenized mycelium of the transformants were used in PCR amplification with primer pair hptF1-hptR1 (a) or gpdF12-lccR12 (b). “M” indicates a 100 bp ladder size marker. “NC” indicates negative control (reactions with no PCR template). “PC” indicates positive control using pGL as PCR template. The PCR products were electrophoresed in 2 % agar and stained with SYBR Green (Takara)