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. 2015 Dec 23;6:1170. doi: 10.3389/fpls.2015.01170

FIGURE 4.

FIGURE 4

BLSVP8d solubility and stability in fresh and lyophilized leaves. 12.5% SDS-PAGE gels stained with Coomassie brilliant blue showing total (T), soluble (S), and insoluble (I) protein fractions from: (A) 2 mg of transplastomic WT, BLSVP8d, and VP8∗ leaves extracted in Laemmli buffer. VP8∗ was included as an insoluble control. (B) 0.25 mg of transplastomic WT and BLSVP8d leaves from lyophilized leaves stored at -80°C and extracted in Laemmli buffer. Total extracts (3 mg) from wild-type and BLSVP8d fresh leaves were included as controls. (C) 0.25 mg of lyophilized leaves stored at room temperature for one month and extracted in Laemmli buffer. A total extract (2 mg) from a BLSVP8d fresh leaf was included as control. BLSVP8d, VP8∗, RbcL, and molecular weight marker (Broad Range Protein Molecular Weight Markers) positions are indicated on the margins.