Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 23;6:1170. doi: 10.3389/fpls.2015.01170

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Alfano, Lentz, Bellido, Dus Santos, Goldbaum, Wigdorovitz and Bravo-Almonacid.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Detection of specific neutralizing immunoglobulin Y (IgY) antibodies against BLSVP8d after immunization of hens with either fresh or lyophilized transplastomic soluble protein extracts. (A) Detection of specific IgY antibodies in egg yolk obtained from hens immunized with total soluble protein extracts. Titers are indicated both for fresh (dark bars) and lyophilized (light bars) samples. Animals received three doses containing 3.83 μg of BLSVP8d (2 μg corresponded to VP8d). As a control, groups of hens were also immunized with total soluble protein extracts obtained from fresh and lyophilized wild-type leaves. Purified IgY response was evaluated by ELISA. Titers were expressed as the reciprocal of the highest IgY dilution which gave an OD value above the mean values of the respective control samples plus 3 standard deviations. (B) Viral neutralization assay to assess the prevention of bovine rotavirus infection in vitro conferred by specific IgY antibodies. Titers were expressed by the Reed and Muench’s (1938) method considering the highest dilution that resulted in an 80% reduction of fluorescent foci. Titers are indicated both for fresh (dark bars) and lyophilized (light bars) samples. An IgY fraction obtained by immunization of a hen with BLSVP8d previously produced in E. coli was included as control (white bar).