Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 21;4(12):e180. doi: 10.1038/oncsis.2015.37

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Chloroquine inhibits DNA damage-induced centrosome amplification. (a, b) ETO treatment (10 μM, 24 h) induces autophagy in adrenocortical Y1 tumor cells. (a) Immunostaining of autophagosomes with antibody against LC3 in the presence or absence of ETO. DNA is stained with DAPI. (b) Extracts of ETO-treated Y1 cell lysates are analyzed by immunoblotting with antibodies against LC3, γ-H2AX and GAPDH. (c, d) Chloroquine inhibits ETO-induced centrosome amplification (c) and senescence (d) in adrenocortical Y1 tumor cells. Quantitation of Y1 cells containing multiple centrosomes (c) or senescence (d) in the presence or absence of ETO with or without chloroquine. (e) Chloroquine inhibits ETO-induced centrosome amplification in osteosarcoma U2OS cells. Quantitation of U2OS cells containing multiple centrosomes in the presence or absence of ETO with or without chloroquine. All results are expressed as the mean+/−s.d. from at least three independent experiments. *P<0.05, **P<0.01, ***P<0.001.