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. 2015 Oct 23;5:928–941. doi: 10.1016/j.fob.2015.10.004

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Supplementary Fig. S1 — (A) Specific phospholipase A₂ (PLA₂₎ enzyme activity was determined for various fractions obtained from Superdex G-75 fractions (gel filtration) and RP-HPLC fractions (Sepharose C18 and C8). Chromatography of D. russellii russellii crude venom, fraction RV5 and RV6 showed more activity than the other fractions. The enzymatic activity of reverse-phase fractions (RV-F1 to RV-F3) were compared with activity of pure proteins (VipTx-I and VipTx-II), VipTx-II showed more activity than the fractions. (B) Proteins yields per fraction as well as pure proteins (VipTx-I and VipTx-II). Symbol denotes: RV-F-Russell’s viper fraction, VipTx-I – viperatoxin-I/II.