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In vitro fluorescence imaging and bioluminescence imaging of PC3-HRE-GFP/HRE-ODD-luc/tdTomato cells after CoCl2 addition. EGFP (false color), tdTomato, and bioluminescence imaging of control or CoCl2-treated (200 μM for 24 hours) PC3-HRE-GFP/HRE-ODD-luc/tdTomato cell pellets (3 × 106 cells/tube, n = 3). Exposure times were 1 second for EGFP, 0.1 second for tdTomato, and 1 minute for BLI. Data are expressed as mean ± SEM. *P < .05; **P < .01; ***P < .001; ns, P > .05.
