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. 2015 Dec 13;17(12):849–861. doi: 10.1016/j.neo.2015.11.005

Figure 4.

Figure 4

Breast cancer cells migrate through microporous membranes to human bone tissue fragment-conditioned medium. (A) Traditional migration assay design showing MDA-MB-231-fLuc-EGFP cells seeded onto the upper surface of an insert with an 8-μm porous membrane, through which they migrate to into the receiver well containing bone tissue fragment culture supernatant. (B) Postmigration attachment of cells to the bottom of the well. (C) BLI of the receiver plate after removal of the insert following a 20-hour migration period demonstrating enhanced migration of MDA-MB-231-fLuc-EGFP cells into wells containing bone tissue culture supernatant versus control medium (DME-10% FBS). (D) Comparison of migration of four breast cancer cell lines to bone supernatant from THR specimen 61 reveals enhanced migration of MDA-MB-231-fLuc-EGFP cells to bone tissue supernatant versus control medium (*P < .01), whereas enhanced migration was not observed for the other three breast cell lines: SKBR3-fLuc-EGFP, MCF-10A-fLuc-EGFP, and MCF-7-fLuc-EGFP.