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. 2015 Dec 13;17(12):849–861. doi: 10.1016/j.neo.2015.11.005

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

MDA-MB-231-fLuc-EGFP breast cancer cell migration is enhanced in the presence of bone tissue-conditioned medium generated from 14 hip replacement surgical specimens. (A) Enhanced migration of MDA-MB-231-fLuc-EGFP cells to bone tissue culture supernatants (n = 3) versus control medium (n = 3) in experiments performed using bone fragments from 14 THR specimens. (B) Fold increase in migration in the presence of bone-conditioned medium, determined as the ratio of average triplicate BLI signal for migration to bone supernatant over control medium. Overall migration to bone-conditioned medium was significantly greater than to control medium (P = .0014).