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Amplification of AFP mRNA in HepG-2 cells by nested RT-PCR. Lane 1: Negative control. Lanes 2-9: Titration of total RNA extracted from HepG-2 cells. Each lane includes (from left to right) 5, 1, 0.5, 0.05, 0.005, 0.0005, 0.00005, 0.000005 μg total RNA, respectively.
