Table 1.
Inhibitory effect of aminoguanidine and L-NAME on CM stimulated in vitro nitrite production in primary cultured rat hepatocytes
| Dose (μmol·L-1) | n | c (NO2-) (μmol·L-1) | Inhibition rate (%) | Dose (μmol·L-1) | n | c (NO2-) (μmol·L-1) | Inhibition rate (%) |
| Control | 6 | 6.2 ± 1.0 | Control | 6 | 6.5 ± 1.3 | ||
| CM | 16 | 12.3 ± 4.1b | CM | 8 | 12.6 ± 3.7b | ||
| CM + Aminoguanidine | CM + Aminoguanidine | ||||||
| 0.1 | 16 | 9.6 ± 3.9 | 22.0 | 0.1 | 9 | 10.1 ± 1.9 | 19.8 |
| 1 | 16 | 8.4 ± 2.6a | 31.7 | 1 | 9 | 9.4 ± 2.5 | 25.4 |
| 10 | 16 | 6.9 ± 2.8d | 44.0 | 10 | 9 | 8.5 ± 3.0a | 32.5 |
| 100 | 16 | 5.7 ± 1.9d | 53.7 | 100 | 9 | 7.7 ± 2.4a | 38.9 |
| 1000 | 16 | 5.5 ± 2.1d | 55.3 | 1000 | 9 | 7.8 ± 2.8a | 38.1 |
CM (Cytokines mixture): IL-1β 10 KU·L-1, TNF-α 500 KU·L-1, and IFN-γ 100 KU·L-1 plus LPS 10 mg·L-1; Cultured hepatocytes were stimulated in vitro with CM in the absence or presence of inhibitors (aminoguanidine or L-NAME) at various concentrations for 24 h. Amounts of nitrite in the supernatant were assayed 24 h after start of stimulation in vitro. -x ± s, n = 6-16 rats (3 well for each treatment in each experiment); bP < 0.01, compared with control, aP < 0.05, dP < 0.01 compared with corresponding CM.