Table 4.
Antisense (μmol/L) | Sense (μmol/L) | Hepatoma cell growth (absorbance at 570 nm) | Suppression (%) |
0 | 0 | 1.00 ± 0.08 | |
0 | 10 | 0.95 ± 0.07 | 5 |
10 | 0 | 0.46 ± 0.02 | 54 |
10 | 10 | 0.51 ± 0.05 | 49 |
10 | 100 | 0.95 ± 0.05 | 5 |
SMMC-7721 cells were incubated with combined AFP sense and antisense S-ODNs for 48 h in vitro. The cell growth suppression induced by antisense S-ODNs was reversed by hybridization competition of sense S-ODNs. Each value represents mean ± SD of 3 separate experiments.