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. 2015 Nov 17;28(12):2419–2425. doi: 10.1021/acs.chemrestox.5b00432

Figure 1.

Figure 1

Inhibition of AS3MT activity by TPIMS inhibitors. Methyltranserase activity was assayed with CrAS3MT using the TR-FRET method, as described under Materials and Methods. As(III) was added at 10 μM, SAM was added at 20 μM, and small molecule compounds were added at 50 μM, final concentrations. The reaction was initiated by the addition of enzyme at 1 μM, final concentration. The reaction was terminated after 5 min at room temperature, and SAH production analyzed. The data are the mean ± SE (n = 3). The dotted line indicates 75% inhibition.