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. 2015 Nov 17;28(12):2419–2425. doi: 10.1021/acs.chemrestox.5b00432

Figure 4.

Figure 4

Effect of TPIMS compound binding of As(III) or MAs(III) to AS3MT. Substrate binding was estimated from the quenching of the fluorescence of the single-tryptophan CrArsM Y72W enzyme, as described under Materials and Methods. The 5 μM enzyme was preincubated with individual TPIMS compounds at 50 μM final concentration for 5 min on ice prior to the initiation of the assay with either (A) As(III) or (B) MAs(III) at the indicated concentrations. Black circle, no addition; open circle, TPI-2; inverted black triangle, TPI-4; open triangle, TPI-5; black square, TPI-6. The data are the mean ± SE (n = 3).