Figure 17.
(A) Schematic describing chemical and physical differences between solid-core NPs and water-core polymersomes (PS). Mice were immunized s.c. 3 times at 2-week intervals with various OVA formulations. One week after the final immunization, splenocytes, lymph nodes cells and lung leukocytes were isolated and restimulated ex vivo with OVA for 6 hours. PSs were more effective than NPs in inducing cytokine-secreting CD4+ T-cells (B) while NPs were more effective in inducing cytokine-secreting CD8+ T-cells (C). Co-administration of both particle types elicited T-cell immunity characteristic of the two particles at the same time. Adapted from Stano et al.256 Reprinted with permission from reference 256. Copyright 2013 Elsevier Ltd.