Figure 5.

Different nanoparticle based strategies to improve adoptive T-cell transfer (A) Schematic of iron-dextran nanoparticles functionalized with T-cell activating proteins (nano-APCs) stimulating T-cell receptor signaling in the absence or presence of a magnetic field (B) Adoptive transfer of magnet-enhanced nano-APC activated T cells increased survival compared to no magnet and control groups. Mice were censored if dead or tumors were > 150 mm². (p<0.001 by Mantel Cox log-rank test)⁷⁴ Reprinted with permission from reference 74. Copyright 2014 American Chemical Society. (C) Schematic of maleimide-based conjugation to cell surface thiols. MBP-PE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4– (p-maleimidophenyl)butyamide] (D) Confocal microscopy images of CD8⁺ effector T-cells immediately after conjugation with fluorescent 1,1-dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine (DiD)-labeled multilamellar lipid nanoparticles (top) and after 4 days of in vitro expansion (bottom) (D) Scale bar, 2 µm (E) Survival of mice after adoptive T-cell therapy is enhanced with nanoparticle conjugated T-cells illustrated by Kaplan-Meir curves (n=6 per group)⁷⁹ Reprinted with permission from reference 79. Copyright 2010 Nature America, Inc. (F) T-cell targeted liposome with surface attached anti-Thy1.1 or IL-2Fc (G) Representative histograms of liposomes labeling antigen-specific adoptively transferred or endogenous CD8⁺ T-cells 48 hours after adoptive transfer and 24 hours after liposome injection.⁸³ Reprinted with permission from reference 83. Copyright 2013 Elsevier B.V.