Fig. 3.

BALR-6 overexpression increases proliferation in human Nalm-6 and murine 70Z/3 cells. a qRT-PCR quantitation of BALR-6 isoform expression in Nalm-6 human pre B-ALL cell line. Normalized to ACTIN. b qRT-PCR quantification of BALR-6 isoforms in 70Z/3 mouse pre B-ALL cell line. Normalized to Actin (ISO1) or L32 (ISO3). c Increased cell proliferation in BALR-6 overexpressing Nalm-6 cell lines, as measured by MTS. d Representative histogram of Nalm-6 overexpression lines, stained with propidium iodide, shows an increase in S phase cells and G2-M cells. e, f Increased cell proliferation in BALR-6 Isoform 1 (e) and Isoform 3 (f) overexpressing 70Z/3 cell lines, as measured by MTS. g, h Propidium iodide staining of 70Z/3 cells overexpressing BALR-6 Isoform 1 (g) and Isoform 3 (h), shows a consistent increase in G2-M cells, and a decrease in Sub-G0 cells. Representative histogram of figures (g-h) confirmed the increase in cells in the G2-M when compared to the empty vector, shown to the right. ISO1, Isoform 1; ISO2, Isoform 2; ISO3, Isoform 3. Evaluations were made using a two-tailed T-test, p < 0.05 (*); p < 0.005 (**); p < 0.0005 (***); p < 0.0001 (****)