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. 2015 Dec 22;9:24. doi: 10.1186/s13036-015-0022-z

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© Gnanasekaran et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Metabolite analysis of infiltrated plant leaves. a Total Ion Chromatograms (TIC) for the detection of isopimaradiene using GC-MS analysis. Samples were spiked with the internal standard Eicosene (ISTD). b Mass spectra of isopimaradiene detected in the standard and diTPS-ISO infiltrated leaf extracts. c Extracted ion chromatograms (m/z −303[M + H]⁺, 325[M + Na]⁺, 347 [M + 2Na]⁺) for the detection of isopimaric acid using LC-MS analysis. Isopimaric acid is detected at retention time 10 min. d Mass spectra of isopimaric acid detected in the standard and CYP720B4 + diTPS-ISO infiltrated leaf extracts