Fig. 1.

Schematic overview of the oligonucleotides-linkers-mediated DNA assembly (OLMA) method. During the assembly process, each large fragment was cloned into a standard Donor vector, while double-stranded oligonucleotides as linker were obtained by annealing two complementary ssDNA and generating two proper overhangs (e.g. 0-R and 1-L as left and right overhangs of the first oligo respectively). The large fragments and the receptor vector were bridged by the overhangs of the Oligo-linkers in a single Golden-Gate assembly reaction