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. 2015 Dec 22;9:23. doi: 10.1186/s13036-015-0021-0

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© Zhang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Optimization of lycopene synthetic pathway using OLMA. a The metabolic pathway from IPP&DMAPP to lycopene in E. coli. (b) OLMA was used to optimize RBS, coding sequence and gene order to fine-tune lycopene synthetic pathway