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. 2015 Dec 22;9:23. doi: 10.1186/s13036-015-0021-0

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© Zhang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The results of individual modulation of the RBSs, coding sequences and gene order by OLMA. a RBS library was constructed using the OLMA method. 10–20 RBSs were chosen for each gene of PanEBI with crtE-I-B order. (b) Coding sequence library was constructed by the OLMA method. CrtEBI from 4 species were assembled as crtE-I-B order with the medium strength RBSs E9_6689, B1_5557, I3_3088 and id1_10974. (c) Gene order library was constructed using the OLMA method. The PanE, PanB and PanI genes were assembled in all six orders. The dynamic range and c-factor of each library were shown in the figures