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Construction efficiency of the assembly of LacZ gene using OLMA method
| Plasmids | Number of pieces to assemble | Total colonies(cfu/ug input DNA) | Positive rate (%) |
|---|---|---|---|
| lacZ | 1 | 104 | 99.9 |
| Z1 + Z2 + Z3 | 3 | 103 | 95.0 |
| Z1 + Z2 + Z3 + Z4 | 4 | 500 | 43.0 |
| Z1 + Z2 + Z3 + Z4 + Z5 | 5 | 10 | 10.0 |
