Table 3.
Name | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Yield |
---|---|---|---|---|---|---|---|---|---|
Order | RBS for crtE | RBS for crtI | RBS for crtB | RBS for idi | crtE | crtI | crtB | ||
pLY116 | BIE | E3_(1813) | I6_(1137) | B8_(2059) | id12_(3867) | PanE | PanI | PanB | 15.17 |
pLY111 | BEI | E13_(13670) | I6_(1137) | B1_(5557) | id16_(135699) | PanE | PanI | PanB | 14.69 |
pLY108 | EBI | E9_(6689) | I4_(1630) | B13_(51518) | id3_(5078) | PvaE | PanI | PanB | 13.86 |
pLY101 | BEI | E3-(1813) | I10_(3562) | B2_(1147) | id15_(115818) | RspE | PanI | PanB | 12.81 |
pLY106 | EBI | E9_(6689) | I10_(3562) | B2_(1147) | id6_(4010) | PvaE | PanI | PanB | 12.53 |
pLY112 | BEI | E16_(13174) | I6_(1137) | B11_(25607) | id14_(31121) | PvaE | PanI | RspB | 12.39 |
pLY107 | EBI | E9_(6689) | I6_(1137) | B14_(46327) | id15_(115818) | PvaE | PagI | PanB | 12.07 |
pLY103 | BIE | E14_(5844) | I15_(28086) | B8_(2059) | id14_(31121) | RspE | PanI | PanB | 11.69 |
pLY109 | EIB | E3_(1813) | I6_(1137) | B4_(3088) | id1_(10974) | PanE | PanI | RspB | 11.33 |
pLY102 | BEI | E2_(2472) | I11_(340) | B8_(2059) | id12_(3867) | RspE | PanI | PanB | 11.29 |
Control | EIB | (17707) | (10318) | (10793) | (31786) | PanE | PanI | PanB | 5.54 |
*The numbers with in the parenthese are the theoretical strength of each gene acquired by RBS calculator. The RBS sequences of crtEBI and idi of the Control strain are all “aaggagatata”