. 2015 Dec 22;6:55. doi: 10.1186/s40104-015-0059-3

Table 1.

Primers used for mutation detecting, cloning and expression survey

Primer	Sequences (5′–3′)	Size, bp	Tm, °C
P-BglII	F: TTTACCAATAGCCCACAG	229	53
P-BglII	R: CATTCTGCCTACTGAAATG	229	53
P-HGF-CDS	F: TCTGAGTCGGAAGAGGGT	2, 250	54
P-HGF-CDS	R: ATAAGGCACCACAGTTGTAG	2, 250	54
P-Q-HGF	F: ACCAATGTGCCAATAGATG	229	60
P-Q-HGF	R: TTAGTGATAGATACCGTCCC	229	60
P-qHGF-W	F: GTATCATTGGTAAAGGCGGTAG	119	60
P-qHGF-W	R: ATAGCTCGAAGGCAAAAAG	119	60
P-qHGF-M	F: GTATCATTGGTAAAGGCGGTAG	109	60
P-qHGF-M	R: CCCCGATAGCTGTGTTCG	109	60
P-Q-GAPDH	F: TGTTTGTGATGGGCGTGAACCA	154	60
P-Q-GAPDH	R: ATGGCGTGGACAGTGGTCATAA	154	60
P-HGF-AS	F: TTGGTAAAGGCGGTAGCT	205/190	58
P-HGF-AS	R: TAGCGTACCTCTGGATTGCT	205/190	58