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. 2015 Dec 22;6:258. doi: 10.1186/s13287-015-0223-x

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© Kolf et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Lysis of osteoblastic cells. MSC cultures were osteogenically differentiated for 15 days, lysed with DOC or water, and examined microscopically. a-c Phase contrast images of residual matrix after lysis. d-f Fluorescent live (green)/dead (red) merged images. g-i AlzR staining of matrix calcification. j-l blue staining of ALP enzymatic activity. (a, d, g, j) OBCs before lysis. (b, e, h, k) OBCs after DOC lysis. (c, f, i, l) OBCs after water lysis. Microscopy images taken at 10×. MSC mesenchymal stem cells, AlzR Alizarin Red S, ALP alkaline phosphatase, OBCs osteoblastic cells